Difference between revisions of "Part:BBa K5226017"
 
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<partinfo>BBa_K5226017 short</partinfo>
 
<partinfo>BBa_K5226017 short</partinfo>
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===Sequence and Features===
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<partinfo>BBa_K5226017 SequenceAndFeatures</partinfo>
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<br>
  
 
===Introduction===
 
===Introduction===
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Mmp1 is an inducible promoter that was designed and characterized by Zhao H (2017)[1] for Halomonas. T7 Promoters are promoters that work with T7 RNA Polymerase.The chromosome of Halomonas sp. TD we used already integrated the RNAP module of MmP1, which was under the control of a lacI-regulated Ptac promoter, named the TD-Mmp1 platform strain. Meanwhile, the promoter module of MmP1 was cloned into pSEVA321 to obtain a PT7-like-p321 plasmid, and then transferred into TD-Mmp1 through conjugation.In order to reduce the leakage level of the Mmp1 promoter, one more lacO was introduced immediately after the transcription start site (+1) of PT7-like (producing PT7-like-LacO).The Mmp1 inducible promoter currently used by our team has undergone various optimizations as described above.
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Mmp1 is an <b>inducible promoter</b> that was designed and characterized by Zhao H (2017)[1] for <i>Halomonas</i>. T7 Promoters are promoters that work with T7 RNA Polymerase.<b>The chromosome of <i>Halomonas sp.</i> TD we used already integrated the RNAP module of MmP1</b>, which was <b>under the control of a lacI-regulated Ptac promoter</b>, named the TD-Mmp1 platform strain. Meanwhile, the promoter module of MmP1 was cloned into pSEVA321 to obtain a PT7-like-p321 plasmid, and then transferred into TD-Mmp1 through conjugation.
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<br>
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===Sequence and Features===
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In order to <b>reduce the leakage level of the Mmp1 promoter</b>, <b>one more lacO</b> was introduced immediately after the transcription start site (+1) of PT7-like (producing PT7-like-LacO).The Mmp1 inducible promoter currently used by our team has undergone various optimizations as described above.
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<partinfo>BBa_K5226017 SequenceAndFeatures</partinfo>
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===References===
 
===References===

Latest revision as of 09:35, 13 September 2024

Mmp1 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

Mmp1 is an inducible promoter that was designed and characterized by Zhao H (2017)[1] for Halomonas. T7 Promoters are promoters that work with T7 RNA Polymerase.The chromosome of Halomonas sp. TD we used already integrated the RNAP module of MmP1, which was under the control of a lacI-regulated Ptac promoter, named the TD-Mmp1 platform strain. Meanwhile, the promoter module of MmP1 was cloned into pSEVA321 to obtain a PT7-like-p321 plasmid, and then transferred into TD-Mmp1 through conjugation.
In order to reduce the leakage level of the Mmp1 promoter, one more lacO was introduced immediately after the transcription start site (+1) of PT7-like (producing PT7-like-LacO).The Mmp1 inducible promoter currently used by our team has undergone various optimizations as described above.

References

[1]Zhao H, Zhang H M, Chen X, et al. Novel T7-like expression systems used for Halomonas[J]. Metabolic engineering, 2017, 39: 128-140.