Difference between revisions of "Part:BBa K5330020"
m
Line 18: Line 18:
 
<partinfo>BBa_K5330020 parameters</partinfo>
 
<partinfo>BBa_K5330020 parameters</partinfo>
 
<!-- -->
 
<!-- -->
−
<html><img src=“https://static.igem.wiki/teams/5330/registry-parts/screenshot-2024-05-20-at-12-41-22-pm.png” width=“100%”></html>
+
<html>
 +
<center><img src = "https://static.igem.wiki/teams/5330/registry-parts/screenshot-2024-05-20-at-12-41-22-pm.png"></center>
 +
</html>

Revision as of 09:53, 12 September 2024


SmBiT-Encapsulin2A

This part along with BBa_K5330021 are the two components of a test for Mycobacterium avium subspecies paratuberculosis (MAP.) This part is composed of a type 2A encapsulin (from MAP) with a linker to a SmBiT. The SmBiT is half of a split luciferase. This acts as a reporter system for cage formation of Encapsulins linked to either LgBiT or SmBiT which when they come together in the presence of NanoGlo reagents, they produce light. With both SmBiT-Encap2A and LgBiT-Encap2A present in the test, light output is detected. When a blood sample of a cow infected with Johnes diseases is introduced into this solution, there should be a detected loss of light. This is because Encapsulin monomers from MAP will rearrange to incorporate our engineered monomers (SmBiT-Encap2A and LgBiT-Encap2A) resulting in distance being introduced between halves of the split luciferase and either less or no light produced at all.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]