Difference between revisions of "Part:BBa K5330020"

Revision as of 09:46, 12 September 2024

SmBiT-Encapsulin2A

This part along with BBa_K5330021 are the two components of a test for Mycobacterium avium subspecies paratuberculosis (MAP.) This part is composed of a type 2A encapsulin (from MAP) with a linker to a SmBiT. The SmBiT is half of a split luciferase. This acts as a reporter system for cage formation of Encapsulins linked to either LgBiT or SmBiT which when they come together in the presence of NanoGlo reagents, they produce light. With both SmBiT-Encap2A and LgBiT-Encap2A present in the test, light output is detected. When a blood sample of a cow infected with Johnes diseases is introduced into this solution, there should be a detected loss of light. This is because Encapsulin monomers from MAP will rearrange to incorporate our engineered monomers (SmBiT-Encap2A and LgBiT-Encap2A) resulting in distance being introduced between halves of the split luciferase and either less or no light produced at all.

Sequence and Features