Difference between revisions of "Part:BBa K5036002"
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==Usage== | ==Usage== | ||
This part is attached to dCas9(C) so Upon dimerization of the two domains of dCas9 , NLS will direct the dCas9-VP64 complex to the nucleus | This part is attached to dCas9(C) so Upon dimerization of the two domains of dCas9 , NLS will direct the dCas9-VP64 complex to the nucleus | ||
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/parts/nls-attia.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of nuclear localization signal (NLS) | ||
+ | . </span></p></div></html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 06:51, 12 September 2024
NLS
Part Description
It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) to keep it in the nucleus to avoid dCas9 self assembly.
Usage
This part is attached to dCas9(C) so Upon dimerization of the two domains of dCas9 , NLS will direct the dCas9-VP64 complex to the nucleus
this figure illustrates the structure of nuclear localization signal (NLS) .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]