Difference between revisions of "Part:BBa K5398006"
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<P>To generate the squid ring proteins with various long tandem repeats (TRn), we designed a circular mRNA to repeatedly translate the sequence of TRn5. </p>
 
<P>To generate the squid ring proteins with various long tandem repeats (TRn), we designed a circular mRNA to repeatedly translate the sequence of TRn5. </p>
 
<p>If you want to know more about the mechanism of cmRNA, please click the link above. https://parts.igem.org/Part:BBa_K5398002 </p>
 
<p>If you want to know more about the mechanism of cmRNA, please click the link above. https://parts.igem.org/Part:BBa_K5398002 </p>
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 +
<p>We tried different strategies for protein production and purification using cmRNA, and tested its function. </p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:30, 10 September 2024


pET29a(+)-cmRNA

To generate the squid ring proteins with various long tandem repeats (TRn), we designed a circular mRNA to repeatedly translate the sequence of TRn5.

If you want to know more about the mechanism of cmRNA, please click the link above. https://parts.igem.org/Part:BBa_K5398002

We tried different strategies for protein production and purification using cmRNA, and tested its function.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 308
    Illegal XhoI site found at 861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]