Difference between revisions of "Part:BBa K5226074:Design"

 
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===Source===
 
===Source===
  
−
PCR amplification.The gene cluster is directly obtained from the laboratory.
+
PCR amplification.Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009977.1）
  
 
===References===
 
===References===

Latest revision as of 08:41, 10 September 2024


B0064-porin281-Vib-ftl-porin194-Vib-folD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1189
    Illegal NheI site found at 2375
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1189
    Illegal NgoMIV site found at 455
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

PCR amplification.Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009977.1）

References