Difference between revisions of "Part:BBa K180008:Design"
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pmCherry vector, Clontech | pmCherry vector, Clontech | ||
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===References=== | ===References=== | ||
Shaner et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein. Nat Biotechnol (2004) vol. 22 (12) pp. 1567-1572 | Shaner et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein. Nat Biotechnol (2004) vol. 22 (12) pp. 1567-1572 | ||
Revision as of 11:08, 21 October 2009
mCherry (rights owned by Clontech)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 352
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 352
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 352
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 352
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.
N.B. This part has not been submitted to the registry, as the rights to mCherry are commercially owned by Clontech. Academic researchers are welcome to use this part, subject to a material transfer agreement from the supplying university, providing that the DNA will not be used for commercial profit or gain. The sequence provided on this registry page has been provided with express permission from Clontech.
Source
pmCherry vector, Clontech
References
Shaner et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein. Nat Biotechnol (2004) vol. 22 (12) pp. 1567-1572