Difference between revisions of "Part:BBa K180008"
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| − | A DsRed fluorescent protein derivative that can be used as a fluorescent reporter for biological applications. mCherry has a relatively short maturation time at 37C, however only has 27% brightness of fully mature DsRed. | + | A DsRed fluorescent protein derivative that can be used as a fluorescent reporter for biological applications. mCherry has a relatively short maturation time at 37C (15 minutes), however only has 27% brightness of fully mature DsRed. |
Latest revision as of 11:05, 21 October 2009
mCherry (rights owned by Clontech)
mCherry fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through specially designed primers.
N.B. This part has not been submitted to the registry, as the rights to mCherry are commercially owned by Clontech. Academic researchers are welcome to use this part, subject to a material transfer agreement from the supplying university, providing that the DNA will not be used for commercial profit or gain. The sequence provided on this registry page has been provided with express permission from Clontech.
Usage and Biology
A DsRed fluorescent protein derivative that can be used as a fluorescent reporter for biological applications. mCherry has a relatively short maturation time at 37C (15 minutes), however only has 27% brightness of fully mature DsRed.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 352
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 352
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 352
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 352
- 1000COMPATIBLE WITH RFC[1000]