Difference between revisions of "Part:BBa K5036005"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5036005 short</partinfo> | <partinfo>BBa_K5036005 short</partinfo> | ||
− | + | ==Part Description== | |
It is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447*: DALDDFDLDML) connected with glycine-serine (GS) linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator | It is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447*: DALDDFDLDML) connected with glycine-serine (GS) linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator | ||
+ | ==Usage== | ||
+ | This part when fused with GAL4 it acts as a powerful transcription activator where it induces increased gene expression so therefore it increases expression of YAP. | ||
+ | |||
+ | ==literature characterization== | ||
+ | vp64 were transfected into HEK293T reporter cells and treated with abscisic acid or DMSO for 48 h. | ||
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Revision as of 17:44, 9 September 2024
VP64
Part Description
It is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447*: DALDDFDLDML) connected with glycine-serine (GS) linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator
Usage
This part when fused with GAL4 it acts as a powerful transcription activator where it induces increased gene expression so therefore it increases expression of YAP.
literature characterization
vp64 were transfected into HEK293T reporter cells and treated with abscisic acid or DMSO for 48 h.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]