Difference between revisions of "Part:BBa K174003:Design"
Line 18: Line 18:
 
#Haynes, K., M. Broderick, et al. (2008). "Engineering bacteria to solve the Burnt Pancake Problem." Journal of Biological Engineering 2(1): 8.  
 
#Haynes, K., M. Broderick, et al. (2008). "Engineering bacteria to solve the Burnt Pancake Problem." Journal of Biological Engineering 2(1): 8.  
 
#Kutsukake, K., et al., Two DNA Invertases Contribute to Flagellar Phase Variation in Salmonella enterica Serovar Typhimurium Strain LT2. J. Bacteriol., 2006. 188(3): p. 950-957.
 
#Kutsukake, K., et al., Two DNA Invertases Contribute to Flagellar Phase Variation in Salmonella enterica Serovar Typhimurium Strain LT2. J. Bacteriol., 2006. 188(3): p. 950-957.
 +
#Veening, J.-W., H. Murray, and J. Errington, A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes & Development, 2009. 23(16): p. 1959-1970.

Revision as of 10:43, 21 October 2009

Heritable, Tunable, Stochastic Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1791
    Illegal AgeI site found at 105
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It can be tuned by IPTG and Xylose however if one wishes to control the switch with something else, the control regions can easily be replaced since they are surrounded with restriction sites.


Source

Synthesised

References

  1. Ham, T.S., et al., Design and Construction of a Double Inversion Recombination Switch for Heritable Sequential Genetic Memory. PLoS ONE, 2008. 3(7): p. e2815.
  2. Haynes, K., M. Broderick, et al. (2008). "Engineering bacteria to solve the Burnt Pancake Problem." Journal of Biological Engineering 2(1): 8.
  3. Kutsukake, K., et al., Two DNA Invertases Contribute to Flagellar Phase Variation in Salmonella enterica Serovar Typhimurium Strain LT2. J. Bacteriol., 2006. 188(3): p. 950-957.
  4. Veening, J.-W., H. Murray, and J. Errington, A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes & Development, 2009. 23(16): p. 1959-1970.