Difference between revisions of "Part:BBa K180001:Design"
(→References) |
(→References) |
||
| Line 17: | Line 17: | ||
A reference for the original part E0040 (GFPmut3b): | A reference for the original part E0040 (GFPmut3b): | ||
| + | |||
Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8 | Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8 | ||
Latest revision as of 10:43, 21 October 2009
Green fluorescent protein (+LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Design Notes
LVA degradation tail and prefix/suffix addition by sequential PCR amplifications with specially designed primers.
Source
Based upon GFP from E0040, with LVA degradation tail attached.
References
A reference for the original part E0040 (GFPmut3b):
Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8