Difference between revisions of "Part:BBa K5398006"

Revision as of 04:33, 7 September 2024

pET29a(+)-cmRNA

To generate the squid ring proteins with various long tandem repeats (TRn), we designed a circular mRNA to repeatedly translate the sequence of TRn5.

If you want to know more about the mechanism of cmRNA, please click the link above. https://parts.igem.org/Part:BBa_K5398002

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 308
Illegal XhoI site found at 861
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5398006 short</partinfo>
-To generate the squid ring proteins with various long tandem repeats (TRn), we utilized the td intron, an intron of the td gene from T4 phage belonging to group IE, which circularizes the exon to form a back-splice junction (BSJ) in a reaction catalyzed by guanosine. To ensure that the ribosomes do not translate the ORF of TRn from unprocessed linear mRNA, the ribosome binding sequence (RBS) and translation initiation codon ATG were placed downstream of the TRn coding sequence. Consequently, the regulatory sequences were located upstream of the coding sequence only after circularization of the mRNA. To purify the resulting TRn polypeptides, a His tag was incorporated into the ORF. If the mRNA is circularized, the ribosome could circle the cmRNA, producing a long repeating polypeptide.
+<P>To generate the squid ring proteins with various long tandem repeats (TRn), we designed a circular mRNA to repeatedly translate the sequence of TRn5. </p>
+<p>If you want to know more about the mechanism of cmRNA, please click the link above. https://parts.igem.org/Part:BBa_K5398002 </p>
 <!-- Add more about the biology of this part here