Difference between revisions of "Part:BBa K5398005"

 
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In order to obtain proteins with self-healing properties, we used  the pET29a(+) vector to express TRn5.
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We tried different strategies for TRn5 protein production and purification and tested its function.
<partinfo>BBa_K5398005 short</partinfo>
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The part uses the pET29a(+) vector to express TRn5.
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TRn5, composed of squid ring teeth proteins with five tandem repeats, can connect with other squid ring proteins through their common &#946;-sheet.
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Given the positive correlation between number of repeat units and magnitude of cohesive force, we used TRn5 as special materials to realize self-healing.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5398005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K5398005 parameters</partinfo>
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Revision as of 00:50, 7 September 2024

In order to obtain proteins with self-healing properties, we used the pET29a(+) vector to express TRn5. We tried different strategies for TRn5 protein production and purification and tested its function.