Difference between revisions of "Part:BBa K180000:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.
 
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The sequence here is preceeded by a coding region for lacIq; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. When IPTG is no longer present in the system, the promoter will revert to it's non-induced state of 'OFF'.
  
 
===Source===
 
===Source===

Latest revision as of 10:28, 21 October 2009

Hybrid promoter (trp & lac regulated -- tac pR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence here is preceeded by a coding region for lacIq; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. When IPTG is no longer present in the system, the promoter will revert to it's non-induced state of 'OFF'.

Source

pMAL-pIII (New England Biolabs N8101S)

References

[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25