Difference between revisions of "Part:BBa K176016"

Latest revision as of 10:27, 21 October 2009

pCon 0.01(J23103)->RBS+GFP+T

pCon 0.01(J23103)+I13504

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

MEASUREMENT

We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115 )strength by ligated each with a 
GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
K176011: J23100 + GFP ( I13504 )
K176012: J23101 + GFP ( I13504)
K176013: K176009( J23107) + GFP ( I13504)
K176014: K176008( J23115) + GFP ( I13504)
K176015: J23109 + GFP ( I13504)
K176016: J23103 + GFP ( I13504)

Model

To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.

data

J23103 + GFP

COLONY NUMBER	T(min)	OD600	FLU(arbitrary)	ST.FLU(ustc_st.1)	FLU/OD600(arbitrary)	ST.FLU/OD600(ustc_st.1)
Ⅰ	0	0.343	0.218	0.871407443	0.635568513	2.540546481
Ⅰ	30	0.443	0.227	0.90738298	0.51241535	2.048268577
Ⅰ	60	0.519	0.316666667	1.265805919	0.61014772	2.438932406
Ⅰ	90	0.657	0.488666667	1.953338397	0.743784881	2.973117803
Ⅰ	120	0.691	0.577666667	2.309096481	0.835986493	3.341673634
Ⅰ	150	0.907	0.757333333	3.027274786	0.834987137	3.337678927
Ⅰ	180	1.366	1.305	5.216452812	0.95534407	3.818779511
Ⅱ	0	0.323	0.168333333	0.672875778	0.521155831	2.083206742
Ⅱ	30	0.418	0.245666667	0.981998907	0.587719298	2.349279683
Ⅱ	60	0.55	0.350666667	1.401713501	0.637575758	2.548570003
Ⅱ	90	0.621	0.412666667	1.649544976	0.664519592	2.656272103
Ⅱ	120	0.666	0.532	2.126553943	0.798798799	3.193023939
Ⅱ	150	0.888	0.721	2.882040213	0.811936937	3.24554078
Ⅱ	180	1.392	1.289333333	5.15382873	0.926245211	3.702463168

For the details of the definition of ST.FLU and the unit ustc_st1, please click here.

plot

protocol

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

@@ Line 19: / Line 19: @@
   K176015: J23109 + GFP ( I13504)
   K176016: J23103 + GFP ( I13504)
+===Model===
+To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.
 ===data===
 J23103 + GFP