Difference between revisions of "Part:BBa K5198012:Design"

 
 
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pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492 ; http://n2t.net/addgene:85492 ; RRID:Addgene_85492)
 
pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492 ; http://n2t.net/addgene:85492 ; RRID:Addgene_85492)
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===References===
 

Latest revision as of 01:53, 5 September 2024


sfGFP (human codon optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start codon and His-tag were excluded from the sequence.


Source

pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492 ; http://n2t.net/addgene:85492 ; RRID:Addgene_85492)