Difference between revisions of "Part:BBa K176015"

Latest revision as of 10:26, 21 October 2009

pCon 0.04(J23109)->RBS+GFP+T

pCon 0.04(J23109)+I13504

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

MEASUREMENT

We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115 )strength by ligated each with a 
GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
K176011: J23100 + GFP ( I13504 )
K176012: J23101 + GFP ( I13504)
K176013: K176009( J23107) + GFP ( I13504)
K176014: K176008( J23115) + GFP ( I13504)
K176015: J23109 + GFP ( I13504)
K176016: J23103 + GFP ( I13504)

Model

To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.

data

J23109 + GFP

COLONY NUMBER	T(min)	OD600	FLU(arbitrary)	ST.FLU(ustc_st.1)	FLU/OD600(arbitrary)	ST.FLU/OD600(ustc_st.1)
Ⅰ	0	0.383	0.355666667	1.421699911	0.928633594	3.712010211
Ⅰ	30	0.495	0.509333333	2.035948888	1.028956229	4.113028057
Ⅰ	60	0.592	0.614	2.454331055	1.037162162	4.145829485
Ⅰ	90	0.703	0.786	3.141863533	1.118065434	4.469222664
Ⅰ	120	0.746	0.989	3.953311748	1.325737265	5.299345507
Ⅰ	150	0.958	1.309333333	5.233774367	1.366736256	5.463230028
Ⅰ	180	1.338	2.187	8.742055402	1.634529148	6.533673694
Ⅱ	0	0.294	0.229333333	0.916709971	0.780045351	3.118061124
Ⅱ	30	0.399	0.344333333	1.376397383	0.86299081	3.449617502
Ⅱ	60	0.491	0.427666667	1.709504204	0.871011541	3.481678623
Ⅱ	90	0.581	0.572	2.286445217	0.984509466	3.93536182
Ⅱ	120	0.625	0.729	2.914018467	1.1664	4.662429548
Ⅱ	150	0.835	0.964333333	3.854712129	1.15489022	4.616421711
Ⅱ	180	1.213	1.821666667	7.2817151	1.501786205	6.003062737

For the details of the definition of ST.FLU and the unit ustc_st1, please click here.

plot

protocol

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

@@ Line 20: / Line 20: @@
   K176015: J23109 + GFP ( I13504)
   K176016: J23103 + GFP ( I13504)
+==Model==
+To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.
 ===data===
 J23109 + GFP