Difference between revisions of "Part:BBa K5117004:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
removal of restriction sites which are used for RFC10 or TypeIIS standards
+
removal of restriction sites which are used for RFC10 or TypeIIS standards,
 +
exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism
  
  

Revision as of 09:55, 31 August 2024


AtCelG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1539
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC10 or TypeIIS standards, exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism


Source

Acetivibrio thermocellum

References