Difference between revisions of "Part:BBa K5082005:Design"
 
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===References===
 
===References===
 
[1] Fischer, Joseph W et al. “Structure-Mediated RNA Decay by UPF1 and G3BP1.” Molecular cell vol. 78,1 (2020): 70-84.e6. doi:10.1016/j.molcel.2020.01.021
 
[1] Fischer, Joseph W et al. “Structure-Mediated RNA Decay by UPF1 and G3BP1.” Molecular cell vol. 78,1 (2020): 70-84.e6. doi:10.1016/j.molcel.2020.01.021
−
 
 
[2] Ermolenko, Dmitri N, and David H Mathews. “Making ends meet: New functions of mRNA secondary structure.” Wiley interdisciplinary reviews. RNA vol. 12,2 (2021): e1611. doi:10.1002/wrna.1611
 
[2] Ermolenko, Dmitri N, and David H Mathews. “Making ends meet: New functions of mRNA secondary structure.” Wiley interdisciplinary reviews. RNA vol. 12,2 (2021): e1611. doi:10.1002/wrna.1611
−
 
 
[3] Mestre-Fos, Santi et al. “eIF3 engages with 3'-UTR termini of highly translated mRNAs in neural progenitor cells.” bioRxiv : the preprint server for biology 2023.11.11.566681. 11 Nov. 2023, doi:10.1101/2023.11.11.566681. Preprint.
 
[3] Mestre-Fos, Santi et al. “eIF3 engages with 3'-UTR termini of highly translated mRNAs in neural progenitor cells.” bioRxiv : the preprint server for biology 2023.11.11.566681. 11 Nov. 2023, doi:10.1101/2023.11.11.566681. Preprint.
−
 
 
[4] Gaspar, Paulo et al. “mRNA secondary structure optimization using a correlated stem-loop prediction.” Nucleic acids research vol. 41,6 (2013): e73. doi:10.1093/nar/gks1473
 
[4] Gaspar, Paulo et al. “mRNA secondary structure optimization using a correlated stem-loop prediction.” Nucleic acids research vol. 41,6 (2013): e73. doi:10.1093/nar/gks1473
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+
[5] “RNAfold Web Server.” Univie.ac.at, 2024, rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi?PAGE=3&ID=aRk6YHn0WG.
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[5] “RNAfold Web Server.” Univie.ac.at, 2024, rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi?PAGE=3&ID=aRk6YHn0WG
+
−
.
+
 
[6] Ge, Yidong et al. “The roles of G3BP1 in human diseases (review).” Gene vol. 821 (2022): 146294. doi:10.1016/j.gene.2022.146294
 
[6] Ge, Yidong et al. “The roles of G3BP1 in human diseases (review).” Gene vol. 821 (2022): 146294. doi:10.1016/j.gene.2022.146294
−
 
 
[7] Xiong, Rui et al. “G3BP1 activates the TGF-β/Smad signaling pathway to promote gastric cancer.” OncoTargets and therapy vol. 12 7149-7156. 2 Sep. 2019, doi:10.2147/OTT.S213728
 
[7] Xiong, Rui et al. “G3BP1 activates the TGF-β/Smad signaling pathway to promote gastric cancer.” OncoTargets and therapy vol. 12 7149-7156. 2 Sep. 2019, doi:10.2147/OTT.S213728

Latest revision as of 09:28, 31 August 2024


In our project, we found that the G3BP1 protein was overexpressed in gastric cancer (GC) cells [6]. Meanwhile, G3BP1 could bind with HSU structures and lead to mRNA degradation [7]. Therefore, we fused the EIF3B-HSU sequence downstream to reporter genes: GFP and luciferase, to monitor G3BP1 levels and hence diagnose GC. The experimental outline is shown in Figure 2.

eif3b-hsu-2.png Figure 2. Experimental outline. (A) GFP sensor system. (B) Luciferase sensor system.

Design Notes

None


Source

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References

[1] Fischer, Joseph W et al. “Structure-Mediated RNA Decay by UPF1 and G3BP1.” Molecular cell vol. 78,1 (2020): 70-84.e6. doi:10.1016/j.molcel.2020.01.021 [2] Ermolenko, Dmitri N, and David H Mathews. “Making ends meet: New functions of mRNA secondary structure.” Wiley interdisciplinary reviews. RNA vol. 12,2 (2021): e1611. doi:10.1002/wrna.1611 [3] Mestre-Fos, Santi et al. “eIF3 engages with 3'-UTR termini of highly translated mRNAs in neural progenitor cells.” bioRxiv : the preprint server for biology 2023.11.11.566681. 11 Nov. 2023, doi:10.1101/2023.11.11.566681. Preprint. [4] Gaspar, Paulo et al. “mRNA secondary structure optimization using a correlated stem-loop prediction.” Nucleic acids research vol. 41,6 (2013): e73. doi:10.1093/nar/gks1473 [5] “RNAfold Web Server.” Univie.ac.at, 2024, rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi?PAGE=3&ID=aRk6YHn0WG. [6] Ge, Yidong et al. “The roles of G3BP1 in human diseases (review).” Gene vol. 821 (2022): 146294. doi:10.1016/j.gene.2022.146294 [7] Xiong, Rui et al. “G3BP1 activates the TGF-β/Smad signaling pathway to promote gastric cancer.” OncoTargets and therapy vol. 12 7149-7156. 2 Sep. 2019, doi:10.2147/OTT.S213728