Difference between revisions of "Part:BBa K5226034:Design"
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===Design Notes===
 
===Design Notes===
−
Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 297bp base pair of orfZ from a/t to g/c.
+
Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 280bp base pair of orfZ from a/t to g/c.
  
  

Revision as of 13:33, 30 August 2024


orfZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 295


Design Notes

Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 280bp base pair of orfZ from a/t to g/c.


Source

PCR amplification.Sequence source is from Clostridium kluyveri DSM 555, found through NCBI (https://www.ncbi.nlm.nih.gov/nucleotide/CP000673.1?report=genbank&log$=nuclalign&blast_rank=2&RID=CZT79ZA7016&from=3066402&to=3067674)

References