Difference between revisions of "Part:BBa K5198010:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
In progress
+
The tag amino-acid sequence (Ono et al., 2022) was reverse transcribed and codon optimized for expression in mammalian cells.
  
 
===References===
 
===References===
 +
Ono, Kenji, et al. “Signal Sequence-Dependent Orientation of Signal Peptide Fragments to Exosomes.” International Journal of Molecular Sciences, vol. 23, no. 6, Mar. 2022, p. 3137. PubMed Central, https://doi.org/10.3390/ijms23063137.

Latest revision as of 15:27, 23 August 2024


native SEAP tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence should be added to N-terminus of protein cargo to cause secretion.


Source

The tag amino-acid sequence (Ono et al., 2022) was reverse transcribed and codon optimized for expression in mammalian cells.

References

Ono, Kenji, et al. “Signal Sequence-Dependent Orientation of Signal Peptide Fragments to Exosomes.” International Journal of Molecular Sciences, vol. 23, no. 6, Mar. 2022, p. 3137. PubMed Central, https://doi.org/10.3390/ijms23063137.