Difference between revisions of "Part:BBa K5198000:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
The ectodomain sequence was determined based on the annotation in UniProt entry.
+
The sequence contains native CD19 signal tag, but will secrete due to the lack of transmembrane domain. The ectodomain sequence was determined based on the annotation in UniProt entry.
  
 
===Source===
 
===Source===

Revision as of 08:26, 21 August 2024


CD19ecto


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225
    Illegal SapI.rc site found at 61


Design Notes

The sequence contains native CD19 signal tag, but will secrete due to the lack of transmembrane domain. The ectodomain sequence was determined based on the annotation in UniProt entry.

Source

The sequence was created by reverse transcription of human CD19 protein (UniProt: P15391). The ectodomain was codon optimized for expression in human cells.

References