Difference between revisions of "Part:BBa K5387000:Design"
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Revision as of 08:50, 20 August 2024
Design Notes
The sequence has been optimized for expression in E. coli.
Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:
- fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
- rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
Source
This gene is expressed in humans KÄLLA