Difference between revisions of "Part:BBa K5387000:Design"
| Line 1: | Line 1: | ||
| − | |||
| − | |||
| − | |||
| − | |||
===Design Notes=== | ===Design Notes=== | ||
The sequence has been optimized for expression in E. coli. | The sequence has been optimized for expression in E. coli. | ||
Revision as of 08:49, 20 August 2024
Design Notes
The sequence has been optimized for expression in E. coli.
Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:
- fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
- rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
Source
This gene is expressed in humans KÄLLA