Difference between revisions of "Part:BBa K5387000:Design"

 
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The sequence has been optimized for expression in E. coli.  
 
The sequence has been optimized for expression in E. coli.  
  
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The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.
+
Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:
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+
: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
−
 
+
: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
  
 
===Source===
 
===Source===
 +
This gene is expressed in humans KÄLLA
  
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This gene is expressed in humans.
 
  
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===References===
+
==References===

Revision as of 08:48, 20 August 2024


ALOX12B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1789
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
    Illegal BamHI site found at 2070
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454


Design Notes

The sequence has been optimized for expression in E. coli.

Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:

fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'

Source

This gene is expressed in humans KÄLLA


References=