Difference between revisions of "Part:BBa K5387001:Design"
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<partinfo>BBa_K5387001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5387001 SequenceAndFeatures</partinfo>
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===Description===
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ALOXE3 encodes for the protein Epidermis-type lipoxygenase-3 (eLOX-3), an enzyme which oxidizes fatty acids to create fatty acids hydroperoxides [https://medlineplus.gov/genetics/gene/aloxe3/#resources]. The gene codes for one of the enzymes used for [https://2024.igem.wiki/linkoping/ iGEM24_Linkoping] substitution therapy for Ichthyosis.
  
  
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The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.
 
The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.
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Revision as of 08:14, 20 August 2024


ALOXE3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
    Illegal BglII site found at 1541
    Illegal BglII site found at 1646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1920

Description

ALOXE3 encodes for the protein Epidermis-type lipoxygenase-3 (eLOX-3), an enzyme which oxidizes fatty acids to create fatty acids hydroperoxides [1]. The gene codes for one of the enzymes used for iGEM24_Linkoping substitution therapy for Ichthyosis.


Design Notes

The sequence has been optimized for expression in E. coli.

The sequence contains the amino acid sequence "QPPP", which is very difficult for the ribosomes in E. coli to translate. We did not do it, but for future projects we would recommend altering this part of the sequence, either by mutation or when ordering the sequence.


Source

This gene is expressed in humans.

References

https://medlineplus.gov/genetics/gene/aloxe3/#resources