Difference between revisions of "Part:BBa K5226023"

Revision as of 05:45, 18 August 2024

RBS for Vib-folD

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).

Usage and Biology

This part is a native ribosomal binding site of Vib-folD, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of methenyltetrahydrofolate cyclohydrolase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ===Introduction===
-This part is a native ribosomal binding site of TD-sdaA, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of methenyltetrahydrofolate cyclohydrolase..
+One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
 ===Usage and Biology===
-This part is a native ribosomal binding site of TD-sdaA, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of methenyltetrahydrofolate cyclohydrolase.
+This part is a native ribosomal binding site of Vib-folD, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of methenyltetrahydrofolate cyclohydrolase.
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