Difference between revisions of "Part:BBa K5043004:Design"

 
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===Design Notes===
 
===Design Notes===
 
For GoldenBraid cloning internal BsaI, BsmbI restriction sides were removed using alternative codons.
 
For GoldenBraid cloning internal BsaI, BsmbI restriction sides were removed using alternative codons.
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In order to attach histidin-tag part does not include stop codon.
  
  

Latest revision as of 17:58, 17 August 2024


phdF from M. vanbaalenii PYR-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
    Illegal AgeI site found at 470
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For GoldenBraid cloning internal BsaI, BsmbI restriction sides were removed using alternative codons. In order to attach histidin-tag part does not include stop codon.


Source

M. vanbaalenii Pyr-1 genome

References