Difference between revisions of "Part:BBa K5043001"

Latest revision as of 17:53, 17 August 2024

nidA from M. vanbaalenii Pyr-1

nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyses the oxidation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.

This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria. It was also reported that nidAB is able to oxidize other high molecular weight polycyclic aromatic hydrocarbons. [1]

[1] O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 760
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 760
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 752
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 760
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 760
Illegal NgoMIV site found at 96
Illegal NgoMIV site found at 399
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria.
-It was also reported, that nidAB is able to oxydize other high molecular weight polycyclic aromatic hydrocarbons. [1]
+It was also reported that nidAB is able to oxidize other high molecular weight polycyclic aromatic hydrocarbons. [1]
 [1]	O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10