Difference between revisions of "Part:BBa K5043001"
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<partinfo>BBa_K5043001 short</partinfo>
 
<partinfo>BBa_K5043001 short</partinfo>
  
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nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyzes the oxydation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.  
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nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyses the oxidation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.  
  
  

Revision as of 17:52, 17 August 2024


nidA from M. vanbaalenii Pyr-1

nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyses the oxidation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.


This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria. It was also reported, that nidAB is able to oxydize other high molecular weight polycyclic aromatic hydrocarbons. [1]

[1] O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 752
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 760
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]