Difference between revisions of "Part:BBa K5043003:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
For GoldenBraid cloning internal BsaI, BsmbI restriction sides were removed using alternative codons.
+
For GoldenBraid cloning internal BsaI, BsmbI restriction sides were removed using alternative codons. To attach histidin-tag, no stop codon is included.
  
  

Latest revision as of 17:22, 17 August 2024


phdE from Nocardioides sp. strain KP7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 329
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 658
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For GoldenBraid cloning internal BsaI, BsmbI restriction sides were removed using alternative codons. To attach histidin-tag, no stop codon is included.


Source

Derived from Nocardioides sp. strain KP7 genome

References