Difference between revisions of "Part:BBa K5043001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria. | ||
+ | nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyzes the oxydation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene. | ||
+ | |||
+ | It was also reported, that nidAB is able to oxydize other high molecular weight polycyclic aromatic hydrocarbons. [1] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 16:48, 17 August 2024
nidA from M. vanbaalenii Pyr-1
nidA forms together with nidB, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyzes the oxydation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 760
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 760
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 752
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 760
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 760
Illegal NgoMIV site found at 96
Illegal NgoMIV site found at 399 - 1000COMPATIBLE WITH RFC[1000]