Difference between revisions of "Part:BBa K5124003:Design"

 
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===Design Notes===
 
===Design Notes===
−
Researched them and considered the folding
+
Researched them and found them present in the M.bovis genome.
  
  

Revision as of 14:53, 15 August 2024


Cas12a RD4_target_a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Researched them and found them present in the M.bovis genome.


Source

Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection.

References