Difference between revisions of "Part:BBa K5124002:Design"

 
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===Design Notes===
 
===Design Notes===
−
Researched them and considered the folding
+
Researched them and found them present in the M.bovis genome
  
  

Latest revision as of 14:51, 15 August 2024


Cas12a EthA_target_b_c


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
    Illegal NgoMIV site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Researched them and found them present in the M.bovis genome


Source

Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection.

References