Difference between revisions of "Part:BBa K5124001:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K5124000 short</partinfo>
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<partinfo>BBa_K5124001 short</partinfo>
  
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<partinfo>BBa_K5124000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5124001 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Histidine tags added for protein purification.
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6xHis tag added for protein purification.
  
  

Latest revision as of 14:41, 15 August 2024


LwCas13a codon opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2114
    Illegal BglII site found at 3266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

6xHis tag added for protein purification.


Source

From Leptotrichia wadei Cas13, was originally identified in Adbudayyeh et al 2017.

References