Difference between revisions of "Part:BBa K5226020"
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<partinfo>BBa_K5226020 short</partinfo> | <partinfo>BBa_K5226020 short</partinfo> | ||
| + | ===Introduction=== | ||
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:). | One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part is a native ribosomal binding site of TD-gcvH, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of glycine cleavage system protein H. | ||
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Revision as of 06:24, 13 August 2024
RBS for TD-gcvH
Introduction
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
Usage and Biology
This part is a native ribosomal binding site of TD-gcvH, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of glycine cleavage system protein H.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]