Difference between revisions of "Part:BBa K5088001"
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<partinfo>BBa_K5088001 short</partinfo> | <partinfo>BBa_K5088001 short</partinfo> | ||
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− | < | + | __NOTOC__ |
− | === | + | <partinfo>BBa_K5088001 short</partinfo> |
+ | __TOC__ | ||
+ | |||
+ | <html> | ||
+ | <style> | ||
+ | .mw-body a.external, .link-https { padding-right: 0 } | ||
+ | #bodyContent a[href^="https://"], .link-https { padding-right: 0 } | ||
+ | </style> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | ==Background== | ||
+ | ===Motivation=== | ||
+ | ====Use Cases==== | ||
+ | ====Lack of Endogenous Regulatory Parts==== | ||
+ | ====Political Context==== | ||
+ | |||
+ | ===Plant Synthetic Biology=== | ||
+ | ====Gene Structure in Plants==== | ||
+ | ====Cis-Regulatory Elements==== | ||
+ | |||
+ | ==Design and Characterization== | ||
+ | ===Identification & Design Strategy=== | ||
+ | |||
+ | ==Genetic Context of the Part== | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <html> | ||
+ | </a> | ||
+ | <figure> | ||
+ | <a href='https://static.igem.wiki/teams/5088/registry/go-chart/rpl28.png' target='_blank'> | ||
+ | <img src='https://static.igem.wiki/teams/5088/registry/go-chart/rpl28.png' width='900px'> | ||
+ | </a> | ||
+ | <figcaption><p><b>Figure X:</b> Lorem ipsum dolor sit amen</figcaption> | ||
+ | </figure> | ||
+ | </html> | ||
+ | |||
+ | ==Measurement== | ||
+ | ===Dual Fluorescence Reporter Assay=== | ||
+ | ====Choice of Reporter Genes==== | ||
+ | ===Transient Transformation=== | ||
+ | ====Leaf Infiltration==== | ||
+ | ====Protoplast==== | ||
+ | ===Measurement Setup=== | ||
+ | |||
+ | |||
+ | ==Results== | ||
+ | |||
+ | |||
+ | ==The Dandelion Toolbox== | ||
+ | ===Overview=== | ||
+ | ===Dandelion Handbook=== | ||
+ | |||
+ | ==References== | ||
+ | |||
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Revision as of 11:15, 10 August 2024
Large subunit ribosomal protein L28e - Promoter+5'UTR from T. kok-saghyz
Large subunit ribosomal protein L28e - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 510
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 510
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 510
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 510
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 510
Illegal AgeI site found at 1496 - 1000COMPATIBLE WITH RFC[1000]