Difference between revisions of "Part:BBa K5291007:Design"

(→‎Design Notes)
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===References===
 
===References===
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Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in  survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.
+
[1]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in  survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.
 +
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.

Revision as of 10:30, 10 August 2024


PAO102


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use it to improve the efficiency of carbon delivery between the two engineered bacteria to avoid the harm of CO2 release. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa.

Source

Pseudomonas aeruginosa PAO1

References

[1]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759. [2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.