Difference between revisions of "Part:BBa K5291007"

 
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<partinfo>BBa_K5291007 short</partinfo>
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In our design, the engineered Pseudomonas aeruginosa PAO1 will produce a large amount of CO2 when PE particles are degraded, which can’t be fully absorbed by the mangroves and has the possibility of doing harm to the environments. To make matters worse, the engineered bacteria are able to produce biofilms, where CO2 is hard to deliver. Therefore, we introduce PAO102 into Pseudomonas aeruginosa PAO1 to realize the overexpressing of a kind of alpha-carbonic anhydrase, accelerating the conversion from CO2 to bicarbonate. So the carbon can be transported in the biofilm more easily in the form of bicarbonate, improving the efficiency of carbon delivery from Pseudomonas aeruginosa PAO1 to Rhodopseudomonas palustris CGA009.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5291007 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K5291007 parameters</partinfo>
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Revision as of 10:27, 10 August 2024


PAO102

In our design, the engineered Pseudomonas aeruginosa PAO1 will produce a large amount of CO2 when PE particles are degraded, which can’t be fully absorbed by the mangroves and has the possibility of doing harm to the environments. To make matters worse, the engineered bacteria are able to produce biofilms, where CO2 is hard to deliver. Therefore, we introduce PAO102 into Pseudomonas aeruginosa PAO1 to realize the overexpressing of a kind of alpha-carbonic anhydrase, accelerating the conversion from CO2 to bicarbonate. So the carbon can be transported in the biofilm more easily in the form of bicarbonate, improving the efficiency of carbon delivery from Pseudomonas aeruginosa PAO1 to Rhodopseudomonas palustris CGA009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]