Difference between revisions of "Part:BBa K5291006:Design"
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===References===
 
===References===
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Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is  essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966.
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[1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is  essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966.
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Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.
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[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.

Revision as of 10:04, 10 August 2024


acaP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris.


Source

Rhodopseudomonas palustris CGA009

References

[1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966. [2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.