Difference between revisions of "Part:BBa K5291006:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The DNA sequence is from Rhodopseudomonas palustris CGA009, which ensures its normal expression.
+
The acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris.
  
  
Line 16: Line 16:
  
 
===References===
 
===References===
 +
Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is  essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966.
 +
Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.

Revision as of 10:03, 10 August 2024


acaP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris.


Source

Rhodopseudomonas palustris CGA009

References

Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966. Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.