Difference between revisions of "Part:BBa K5291007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We use it to improve the efficiency of carbon delivery between the two engineered bacteria. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa. | + | We use it to improve the efficiency of carbon delivery between the two engineered bacteria to avoid the harm of CO2 release. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa. |
===Source=== | ===Source=== | ||
Revision as of 10:00, 10 August 2024
PAO102
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 153
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use it to improve the efficiency of carbon delivery between the two engineered bacteria to avoid the harm of CO2 release. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa.
Source
Pseudomonas aeruginosa PAO1
References
Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.