Difference between revisions of "Part:BBa K5291007:Design"

 
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===Design Notes===
 
===Design Notes===
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CO2 produced by Pseudomonas aeruginosa PAO1 during PE degradation cannot be intaken by mangroves and may do harm to the environments. So we need PAO102 to deal with the problem of CO2 release. Moreover, we choose the gene from Pseudomonas aeruginosa PAO1, the engineered bacteria we choose, to ensure the normal expression of the gene.
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We use it to improve the efficiency of carbon delivery between the two engineered bacteria. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa, which ensures its normal expression in the engineered P.aeruginosa.
  
  
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===References===
 
===References===
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Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in  survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.

Revision as of 09:57, 10 August 2024


PAO102


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use it to improve the efficiency of carbon delivery between the two engineered bacteria. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa, which ensures its normal expression in the engineered P.aeruginosa.


Source

Pseudomonas aeruginosa PAO1

References

Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.