Difference between revisions of "Part:BBa K5143014"
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mRuby 2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. | mRuby 2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. | ||
| − | Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (Part: | + | Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (Part:BBa_J63005). |
The excitation wavelength is 559nm and the emission wavelength is 600nm. | The excitation wavelength is 559nm and the emission wavelength is 600nm. | ||
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Revision as of 19:34, 31 July 2024
mRuby2 : reporter Red Fluorescent Protein
Description
mRuby 2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (Part:BBa_J63005). The excitation wavelength is 559nm and the emission wavelength is 600nm.
Construction
The mRuby2 used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene mRuby2 was synthetized in fusion with the secretion signal AGA2 (Part:BBa_K5143020) in 5' and a 6XHis tag in 3'. See the composite part here : BBa_K5143021.
References
1. Lam, A. et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods 9, 1005–1012 (2012).
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]