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	<h1>Description</h1>		<h1>Description</h1>
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−	ECRIRE DES TRUCS ICI POUR LA DESCRIPTION	+	Venus is a Yellow Fluorescent Protein that was developped to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K431009). The excitation wavelength is 516nm and the emission wavelength is 530nm.
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	<img src="" width="400" alt="NOM PHOTO">		<img src="" width="400" alt="NOM PHOTO">
	<h1>Construction</h1>		<h1>Construction</h1>
	<p>		<p>
−	ECRIRE DES TRUCS ICI POUR LA CONSTRUCTION	+	The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here :
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	<h1>References</h1>		<h1>References</h1>
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−	METTRE LES REF ICI	+	1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).
		+	2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
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Revision as of 19:10, 31 July 2024

Venus : reporter Yellow Fluorescent Protein

Description

Venus is a Yellow Fluorescent Protein that was developped to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K431009). The excitation wavelength is 516nm and the emission wavelength is 530nm.

Construction

The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here :

References

1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002). 2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).

Sequence and Features