Difference between revisions of "Part:BBa K5078000:Design"
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+ | Crozet et al. (2018) Birth of a phototsynthetic chassis: A MoClo toolkit enabling synthetic biology in the microalga Chlamydomonas reinhardtii. ACS Synth Biol. 7: 2074-2086 |
Latest revision as of 17:58, 31 July 2024
Nos Z gene from P. stutzeri
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 541
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1303
Illegal PstI site found at 541
Illegal NotI site found at 145 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1520
Illegal BamHI site found at 467
Illegal XhoI site found at 34
Illegal XhoI site found at 1588 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 541
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 541
Illegal NgoMIV site found at 1075 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Obtained from Chlamycollection.org
Source
Chlamy MoClo kit
References
Crozet et al. (2018) Birth of a phototsynthetic chassis: A MoClo toolkit enabling synthetic biology in the microalga Chlamydomonas reinhardtii. ACS Synth Biol. 7: 2074-2086