Difference between revisions of "Part:BBa K5143021"

 
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    <title>Protein Description</title>
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    <h1>Description</h1>
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    <p>
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        Modifier
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    <p>
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        Modifier
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        <img src="https://static.igem.wiki/teams/5143/bba-k5143021-mruby-microscpoe.png" alt="mRuby AGA2">
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        <div class="image-caption">Figure 1. Modifier</div>
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    <h2>Details of the System Function</h2>
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        Modifer
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    <h1>Construction</h1>
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        The gene encoding the chimeric mRuby2 protein fused to AGA2 and 6HIS-Tag has been optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>. We have placed this coding region downstream of the ADH1promoter to ensure strong constitutive expression. The entire construct has been synthesized.
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      <br>
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        This genetic construct has been cloned into the following plasmid backbone: <a href=""></a><br>
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Resulting in the following integrative plasmid:
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    <h1>References</h1>
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    <p>
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        Modifier
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Revision as of 16:42, 31 July 2024


Ruby fused with AGA2 under the control of the ADH1 promoter

Protein Description

Description

Modifier

Modifier

mRuby AGA2
Figure 1. Modifier

Details of the System Function

Modifer

Construction

The gene encoding the chimeric mRuby2 protein fused to AGA2 and 6HIS-Tag has been optimized for synthesis and expression in Saccharomyces cerevisiae. We have placed this coding region downstream of the ADH1promoter to ensure strong constitutive expression. The entire construct has been synthesized.
This genetic construct has been cloned into the following plasmid backbone:
Resulting in the following integrative plasmid:

References

Modifier

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830