Difference between revisions of "Part:BBa K5143013"
Line 3: Line 3:
 
<partinfo>BBa_K5143013 short</partinfo>
 
<partinfo>BBa_K5143013 short</partinfo>
  
 +
 +
<!DOCTYPE html>
 
<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
Line 25: Line 27:
 
<body>
 
<body>
 
     <h1>Description</h1>
 
     <h1>Description</h1>
−
     <p> Plu1537 is a 14kDa insecticidal toxin protein derived from <i>Photorhabdus luminescens</i> TT01. It exhibits strong toxicity against termites through oral ingestion. With 30% homology to a known Bt toxin, it is believed to function in a similar manner. However, our experiments have shown that this protein has not been effective in termite control.
+
     <p>
 +
        Plu1537 is a 14kDa insecticidal toxin protein derived from <i>Photorhabdus luminescens</i> TT01. It exhibits strong toxicity against termites through oral ingestion. With 30% homology to a known Bt toxin, it is believed to function in a similar manner. However, our experiments have shown that this protein has not been effective in termite control.
 
     </p>
 
     </p>
 
     <p>
 
     <p>
Line 42: Line 45:
 
     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
     <p>
 
     <p>
−
         The Plu1537gene was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae<i>.  
+
         The Plu1537 gene was synthesized and its nucleotide sequence optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>.
 
     </p>
 
     </p>
 
     <h1>References</h1>
 
     <h1>References</h1>
 
     <p>
 
     <p>
−
<a href="https://2015.igem.org/Team:ZJU-China">iGEM ZJU-China 2015</a>
+
        <a href="https://2015.igem.org/Team:ZJU-China">iGEM ZJU-China 2015</a><br>
−
 
+
        M. Li et al., MOL BIOL REP 36, 785 (2009).<br>
−
        <br>
+
        M. S. Kelker et al., PLOS ONE 9, (2014).<br>
−
M. Li et al., MOL BIOL REP 36, 785 (2009).<br>
+
−
M. S. Kelker et al., PLOS ONE 9, (2014).<br>
+
 
     </p>
 
     </p>
 
</body>
 
</body>

Revision as of 18:59, 30 July 2024


Plu1537: A Bt Toxin Against Termites Optimized for Expression in Saccharomyces cerevisiae


<!DOCTYPE html> Protein Description

Description

Plu1537 is a 14kDa insecticidal toxin protein derived from Photorhabdus luminescens TT01. It exhibits strong toxicity against termites through oral ingestion. With 30% homology to a known Bt toxin, it is believed to function in a similar manner. However, our experiments have shown that this protein has not been effective in termite control.

In our project, we are developing a versatile insect trap using the Plu1537 toxin. This toxin is expressed in yeast as part of a co-culture system, where it is fused with a Cellulose-Binding Domain (CBD). The CBD allows the toxin to attach to cellulose produced by a bacterium also present in the co-culture. When termites consume the cellulose, they ingest the toxin as well. This method ensures that the toxin is effectively delivered to the termites through the cellulose, making the trap highly effective and targeted for termite control.

Plu1537
Figure 1: Plu1537 Gene
termite
Figure 2: Isoptera family termite

Construction

The Plu1537 gene was synthesized and its nucleotide sequence optimized for synthesis and expression in Saccharomyces cerevisiae.

References

iGEM ZJU-China 2015
M. Li et al., MOL BIOL REP 36, 785 (2009).
M. S. Kelker et al., PLOS ONE 9, (2014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]