Difference between revisions of "Part:BBa K5143010"
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5143010 short</partinfo>
 
<partinfo>BBa_K5143010 short</partinfo>
Line 8: Line 7:
 
     <meta name="viewport" content="width=device-width, initial-scale=1.0">
 
     <meta name="viewport" content="width=device-width, initial-scale=1.0">
 
     <title>Protein Description</title>
 
     <title>Protein Description</title>
 +
    <style>
 +
        /* Style to center the image */
 +
        .center-image {
 +
            display: block;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
        }
 +
    </style>
 
</head>
 
</head>
 
<body>
 
<body>
 
     <h1>Description</h1>
 
     <h1>Description</h1>
 
     <p>
 
     <p>
−
         ECRIRE DES TRUCS ICI POUR LA DESCRIPTION
+
         The α-mating factor is a peptide hormone produced by the yeast <i>Saccharomyces cerevisiae</i>, which is crucial for the mating process of these yeast cells. Here are the key details about the α-mating factor:
 
     </p>
 
     </p>
−
     <img src="" width="400" alt="NOM PHOTO">
+
    <ul>
 +
        <li><strong>Function:</strong> It is involved in the mating process of <i>S. cerevisiae</i> by being secreted by α-type yeast cells to attract a-type cells, facilitating the mating interaction.</li>
 +
        <li><strong>Production:</strong> Synthesized as a pre-protein that includes a signal peptide and the mature α-mating factor peptide.</li>
 +
        <li><strong>Structure and Processing:</strong>
 +
            <ul>
 +
                <li><strong>Pre-Pro-α-Mating Factor:</strong> Initially synthesized with a signal peptide (pre-region) that directs the nascent protein to the secretory pathway.</li>
 +
                <li><strong>Mature Peptide:</strong> After cleavage of the signal peptide, the remaining peptide is processed into the active α-mating factor.</li>
 +
                <li><strong>Processing:</strong> Includes cleavage of the signal peptide and pro-region to yield the mature peptide.</li>
 +
            </ul>
 +
        </li>
 +
        <li><strong>Secretion:</strong>
 +
            <ul>
 +
                <li><strong>Signal Peptide Function:</strong> Directs the protein to the endoplasmic reticulum (ER), where it is cleaved for passage through the secretory pathway.</li>
 +
                <li><strong>Mature Peptide Secretion:</strong> Transported through the Golgi apparatus and secreted into the extracellular environment.</li>
 +
            </ul>
 +
        </li>
 +
        <li><strong>Applications in Biotechnology:</strong>
 +
            <ul>
 +
                <li><strong>Protein Secretion:</strong> The α-mating factor's signal peptide is used to enhance the secretion of various proteins in yeast by attaching it to a protein of interest.</li>
 +
                <li><strong>Engineering Yeast Strains:</strong> Yeast strains can be engineered for efficient production of heterologous proteins by incorporating the α-mating factor signal peptide into their expression constructs.</li>
 +
            </ul>
 +
        </li>
 +
    </ul>
 +
     <img src="https://static.igem.wiki/teams/5143/bba-k5143009-sc-protein-a.png" width="600" alt="α-mating factor" class="center-image">
 
     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
     <p>
 
     <p>
−
      ECRIRE DES TRUCS ICI POUR LA CONSTRUCTION
+
The Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in Saccharomyces cerevisiae.
−
     </p>
+
The gene exists in 2 versions: which limits homologous recombination in the Saccharomyces cerevisiae genome. The second version can be found here : <a href="https://parts.igem.org/Part:BBa_K5143009" target="_blank">BBa_K5143009</a>
 +
These two versions of the alpha-factor can be found in the following composite part:     </p>
 
     <h1>References</h1>
 
     <h1>References</h1>
 
     <p>
 
     <p>
−
        METTRE LES REF ICI
+
Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984).
 +
Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).
 
     </p>
 
     </p>
 
</body>
 
</body>
 
</html>
 
</html>
 +
  
  

Revision as of 18:25, 30 July 2024

Alpha-Factor secretion signal V2 optimized for expression in Saccharomyces cerevisiae

Protein Description

Description

The α-mating factor is a peptide hormone produced by the yeast Saccharomyces cerevisiae, which is crucial for the mating process of these yeast cells. Here are the key details about the α-mating factor:

  • Function: It is involved in the mating process of S. cerevisiae by being secreted by α-type yeast cells to attract a-type cells, facilitating the mating interaction.
  • Production: Synthesized as a pre-protein that includes a signal peptide and the mature α-mating factor peptide.
  • Structure and Processing:
    • Pre-Pro-α-Mating Factor: Initially synthesized with a signal peptide (pre-region) that directs the nascent protein to the secretory pathway.
    • Mature Peptide: After cleavage of the signal peptide, the remaining peptide is processed into the active α-mating factor.
    • Processing: Includes cleavage of the signal peptide and pro-region to yield the mature peptide.
  • Secretion:
    • Signal Peptide Function: Directs the protein to the endoplasmic reticulum (ER), where it is cleaved for passage through the secretory pathway.
    • Mature Peptide Secretion: Transported through the Golgi apparatus and secreted into the extracellular environment.
  • Applications in Biotechnology:
    • Protein Secretion: The α-mating factor's signal peptide is used to enhance the secretion of various proteins in yeast by attaching it to a protein of interest.
    • Engineering Yeast Strains: Yeast strains can be engineered for efficient production of heterologous proteins by incorporating the α-mating factor signal peptide into their expression constructs.
α-mating factor

Construction

The Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in Saccharomyces cerevisiae. The gene exists in 2 versions: which limits homologous recombination in the Saccharomyces cerevisiae genome. The second version can be found here : BBa_K5143009 These two versions of the alpha-factor can be found in the following composite part:

References

Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984). Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]