Difference between revisions of "Part:BBa K5143009"
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− | + | Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984). | |
+ | Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021). | ||
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Revision as of 18:22, 30 July 2024
Alpha-Factor secretion signal V1, optimized for Saccharomyces cerevisiae
Description
The α-mating factor is a peptide hormone produced by the yeast Saccharomyces cerevisiae, which is crucial for the mating process of these yeast cells. Here are the key details about the α-mating factor:
- Function: It is involved in the mating process of S. cerevisiae by being secreted by α-type yeast cells to attract a-type cells, facilitating the mating interaction.
- Production: Synthesized as a pre-protein that includes a signal peptide and the mature α-mating factor peptide.
- Structure and Processing:
- Pre-Pro-α-Mating Factor: Initially synthesized with a signal peptide (pre-region) that directs the nascent protein to the secretory pathway.
- Mature Peptide: After cleavage of the signal peptide, the remaining peptide is processed into the active α-mating factor.
- Processing: Includes cleavage of the signal peptide and pro-region to yield the mature peptide.
- Secretion:
- Signal Peptide Function: Directs the protein to the endoplasmic reticulum (ER), where it is cleaved for passage through the secretory pathway.
- Mature Peptide Secretion: Transported through the Golgi apparatus and secreted into the extracellular environment.
- Applications in Biotechnology:
- Protein Secretion: The α-mating factor's signal peptide is used to enhance the secretion of various proteins in yeast by attaching it to a protein of interest.
- Engineering Yeast Strains: Yeast strains can be engineered for efficient production of heterologous proteins by incorporating the α-mating factor signal peptide into their expression constructs.
![α-mating factor](https://static.igem.wiki/teams/5143/bba-k5143009-sc-protein-a.png)
Construction
The Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in Saccharomyces cerevisiae. The gene exists in 2 versions: which limits homologous recombination in the Saccharomyces cerevisiae genome. The second version can be found here : BBa_K5143010 These two versions of the alpha-factor can be found in the following composite part:
References
Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984). Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]